Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium.
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چکیده
منابع مشابه
Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium.
Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then...
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Mucous neck cells in the rat stomach which contain neutral mucin were stained with a modified Mayer's hemalum. When alcian blue staining and periodic acid-Schiff (PAS) reaction preceded hematoxylin staining, mucin-containing fundic gland cells could be distinguished in the same tissue sections: surface mucous cells were positive for PAS reaction and/or alcian blue staining, neutral mucin-contai...
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Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspeci...
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Since Steedman ( 1950) has introduced it into histochemistry, alcian blue has been advocated to reveal a staining selectivity which depends upon the presence of acidic groups in mucopolysaccharides (Mowry, 1956; Pearse, 1960). As a plausible ground of this concept the fact has been presented that the alcian blue staining of polysaccharides can be increased in intensity, when acidic groups are i...
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After treatment with Alcian blue at high temperatures, nuclei from chicken blood smears showed an intense staining reaction, which proved to be dependent on the DNA content of chromatin. The possibility that interactions between the phthalocyanine chromophore and unpaired bases accounts for this temperature-dependent staining of chromatin is briefly discussed.
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1989
ISSN: 0021-9746
DOI: 10.1136/jcp.42.8.881